ࡱ>  `\pmtkorn Ba=   =xdA}(8@"1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1Arial1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman1.Times New Roman"$"#,##0_);\("$"#,##0\)!"$"#,##0_);[Red]\("$"#,##0\)""$"#,##0.00_);\("$"#,##0.00\)'""$"#,##0.00_);[Red]\("$"#,##0.00\)7*2_("$"* #,##0_);_("$"* \(#,##0\);_("$"* "-"_);_(@_).))_(* #,##0_);_(* \(#,##0\);_(* "-"_);_(@_)?,:_("$"* #,##0.00_);_("$"* \(#,##0.00\);_("$"* "-"??_);_(@_)6+1_(* #,##0.00_);_(* \(#,##0.00\);_(* "-"??_);_(@_) 0.E+00 0.000 0.0000$[$-409]dddd\,\ mmmm\ dd\,\ yyyy[$-409]d\-mmm\-yyyy;@ 0.000E+00[$-409]d\-mmm\-yy;@                + ) , *          "8@ @ "8@ @ #    "8    # "8  #          "8@@  # "  "x@ @   (@ @ "<@ @ "8  (  (   (   ( @  #8@ @ "8@ @   ,@ @   # #  ( @      #    "@ @    @ @  @ @  @ @  @ @  @ @  @@   @ #@    @  "@  "   #       A"@ A"  A  A  A  "  "@   @  @ A"  @  A     A @ A"  A  A     @ @       A   @ @ @ *  @ @ *  " "@ @ * "@ @ *   (@ @  (@ @  "8@ @     "@ @ *  |@ @   l@@ A"@ @ @ @ @ @ @ @  @ @   ,@ @  "  " " "@ @ "@ @ "@ @ " "@@ 0 ! "@ @ " ! #  #  @ " "@  @ #f@@ #f@ "`f @  @ # "   "  " #  "  " "  #f@@ "f@ "@  " "   `f @ "f@@  f@  f@ "`f @ !  "    f@@  "`f @ "   " " "f@  `f @ "  # " #  # #  "  # " " " #  "      "@  # #@ #          A  "@ @ * 8@ @ 8@ @ 0@ @ 0@ @ 0@ @ "0 8@ @   x@ @ 0 0 0  8 8 "8 8  ( @ @ *   "@ @   "       !  "@ @ *  "@ @ *  1"@ @ * @ @    @ @ * @ @ @ @ *  "@ @ *  "@ @ "@ @   @ @  @ @  "@ @   @ @   (@@  @  (@  (  @     (@  8  8  #8 @ "@ @         "    " " " "         "@ @ "    "  "8@@  "8@  "8 @  !    "   )      "8@ @ ! ! "  *8@@  *8 @ "8@@ "8 @  "<@ @  ! #8@ @  ! +8@@ +8 @  ! #8@@ #8 @   ! 8@ @ @@ *  @ * @ @ * @ @ @ @ * @ @  "  1#@ @  *8@ @ 8@ @  *8@ @  *8@ " *@@ *@ * @ *@ * *   @    @@  @ #f@@ #f@ # " "f@@ "`f @ "@ "  "f@@ "`f @  ! "    "8  "<      !8@ @ !p@ @ *  Normal_Tcid50v21`N InstructionsSummary ReportPhysical Titer by qPCR'1Identity and Purity by SDS PAGE2xSDS PAGE PicturesParticle Titer by ELISAInfectious Titer by GFP"RInfectious Titer by TCID50 TCID50 Trial 1 Worksheet TCID50 Trial 2 Worksheet _TCID50 Trial 3 Worksheet D  " qPhysical Titer by qPCRTrialMean QtyDilutionGC/ mL Performed by:A 450nmInfectious Titer by GFPDays Post-InfectionGFU/ mL(Identity and Purity Analysis by SDS-PAGE Silver StainReducedNative% PurityInfectious Titer by TCID50 Log TCID50SE TCID50/ mL Submitted by: Report Date:0AAV2 Reference Standard Characterization Report Institution:Date:1.7Identify dilutions with 20 to 300 green cells per well.2.`Enter the dilution factor in the labeled space of the table below. For 1e-8 dilutions enter 1e8.3.>Enter the number of green cells in each replicate well below. 4.*Average the number of green cells per well5.GGFU/ mL = average number of green cells per well * 20 * dilution factor6.5Pt:GFU = input vector concentration (pt/ml) GFU/ mL7.0Average appropriate values of GFU/ mL and Pt:GFUTrial:Dilution FactorGFP Cells in 12 replicatesDays PIAveragePt:GFUAverage GFU/ well%Infectious Titer Determination by GFP Log TICD50Trial 1 Vector Input Input subtracted copies per well# >740Neg Ctrl logTCID50SE P:I RatioTrial 2Trial 3$Input Vector Physical Titer by qPCR:7Infectious Titer Detmination by TCID50-PCR Assay Report Institute:PCR Run Informationslope: intercept: r-squared:Ct 1e8 standard:Ct 1e1 standard:Ct NTC:Ct Negative Control:Titer CalculationQty/ mL$Physical Titer Determination by qPCRAVERAGE GC/ mL,Efficiency: 0.5 * 10^(-1/ slope) Reviewed by: Review Date:Pt: GFU!Enter data in high-lighted areas.<Dose #1 is the highest dilution with 100% positive response.[Dose entries should be subsequent dilutions until the first dose with 0% positive response.<Enter log (dilution) as log, i.e. enter 1e-8 dilution as -8.Dose #log (dilution) # Positive # Treated % Positive (Pi + Pi+1) [Pi x (1 - Pi)] n   Volume Inoculm (ml)P = % Positive n = # TreatedTotalmX = log (dilution) X1 = log (dilution) at the first dose d = X1 " X2 ml= volume of inoculum in mlStandard Error log (TCID50)SE log (TCID50)= dx"" [Pi x (1 - Pi)] nlog (TCID50) Calculation  log (TCID50) =  X1" " (Pi + Pi+1)  TCID50/ ml Calculation -log (TCID50)  TCID50/ ml =^ml P:I Ratio ='Input Vector (pt/ ml) TCID50/ ml = SE TCID50/ ml = TCID50/ mlln[-log (TCID50)] SE log (TCID50)  SE TCID50/ ml %$log (TCID50) 95% Confidence Interval  log (TCID50) limit =  log (TCID50)   ()lower log (TCID50) =+ (=upper log (TCID50) =" (lower TCID50/ ml =  lower P:I Ratio =2Input Vector (pt/ ml) upper TCID50/ ml = "$upper TCID50/ ml =  upper P:I Ratio =2Input Vector (pt/ ml) lower TCID50/ ml = "$-Trial 3 TCID50 Calculation by Karber's Method-Trial 2 TCID50 Calculation by Karber's Method-Trial 1 TCID50 Calculation by Karber's Method TCID50/mL1AAV Identity and Purity Determination by SDS-PAGEGel A - Silver Stain, ReducedSampleLaneVP3 darker than VP1 and VP2?Valid?Positive ControlNegative ControlAAV Reference Standard VP1 Present? VP2 Present? VP3 Present?Gel A - Silver Stain, NativeContaminating Protein Present?Protein Standard Pass or FailViral Protein Purity (%) Trial 1 DataSeries Trial 2 Data*Molecular Weights of Contaminating Protein Particles/ mLAverage Particles/ mL%Particle Titer Determination by ELISAParticle Titer by ELISA!Gel B - SYPRO Ruby Stain, Reduced Gel B - SYPRO Ruby Stain, NativeSYPRO Ruby Stain?Instructions for AAV Reference Standard Characterization ReportSummary ReportGeneral Guidelines:YDue to the automatic nature of this report most cells are locked and can not be selected.YCells that require data are high-lighted green. Instructions for each sheet follow below.UValues displayed on this sheet are simply copies of the data entered on other sheets.yUpon review of reported data values the reviewer must enter their name and the date in the space provided below the data.oEnter assay data in appropriate locations indicated by green high-light. Enter names and dates where indicated.Identity and Purity by SDS PAGEdGel B SYPRO Ruby Reduced also asks for % Purity. Enter this value as a number, i.e. enter 90% as 90.Each column of each table poses a Yes/ No question. Enter Yes, No, or NA as appropriate in the green high-lighted cells. Enter names and dates where indicated.&Enter names and dates where indicated.CV %CV%CV (%)CV TCID50/ ml (%)= #Coefficient of Variation TCID50/ mlTCID50 Trial WorksheetskFollow the directions to enter data in the green high-lighted cells. Enter names and dates where indicated.Vector Concentration (GC/ mL)^Data values on this page automatically fill as assay data is entered in its respective space.gFollow the directions to enter data in green high-lighted cells. Enter names and dates where indicated.Positive replicates automatically high-light yellow and the number of positives in each dilution is automatically entered to the right of the tableLog(TCID50), standard error, coefficient of variation, TCID50/ mL, and P:I ratio are automatically entered as each trial worksheet is completed."Vector concentration (GC/ mL)" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered.All calculations automatically complete, are entered in the Infectious Titer by TCID50 page and, subsequently, in the summary report.These instructions are intended to aid in the completion of this largely automated report of assay data associated with the characterization of AAV reference material.kPCR efficiency, GC/ mL, and Average GC/ mL are automatically calculated and reported to the summary report.Dilutions with 20 to 300 GFU/ well are used to automatically calculate GFU/ mL. Averaged GFU/mL is automatically entered in the summary report."Input Vector Physical Titer by qPCR" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered. Pt:GFU ratios are automatically calculated and entered in the summary report."Input Vector Physical Titer by qPCR" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered. Input vector values are automatically calculated.[Save this file as data is entered with a name that includes your institution and your name.bSpaces for institution, technician, reviewer, and all dates are unlocked but are not high-lighted.pOnce this report is completed save it with a name including your institution, your name, and the date submitted.]When all assay data is reported, enter the institution name, submitted by, and date sections.Paste the pre-determined input-subtracted copies per well for each replicate of each dilution into the green high-lighted cells in the same orientation they were in on the PCR plate.lEnter A450, dilution, and particles/ mL, determined according to the protocol, in green high-lighted cells.Dilutions averaging less than 20 GFU/ well are labeled "low" and dilutions averaging greater than 300 GFU/ well are labeled "High".Following the guidelines outlined in the protocol, enter pass of fail in the indicated cell for each gel. This value is automatically entered in the summary report.TAverage Particles/ mL is automatically calculated and entered in the summary report.@AAV Identity and Purity Determination by SDS-PAGE (Gel Pictures) Institutuion:]Using the "Insert" menu above, insert SDS PAGE gel pictures here from your saved image files.[This page< has been left unprotected allowing you to insert, size, and format your pictures.qA suggested loading order has been entered below. Please check this loading order and make corrections as needed.Benchmark Ladder - ReducedNegative Control - Reduced AAV Reference Material - ReducedMarker/ Validation - ReducedBenchmark Ladder - NativeNegative Control - NativeAAV Reference Material - NativeMarker/ Validation - Native+ 7,,T-s.R//(M00G12;344 5 f6 @7 7 79 9+:4;}9<b?y@FDpHK ZMcc:  <=  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"_XX??cU<  ; "   "       "    @  "  @ @ "  @   @  "        ~ ?  ~ @  ~ @ ~ @ ~ @    ~ ? ~  @ ~  @  ~  @  ~  ?  ~ @  ~ ?   ~ @ ~ @   ~ ? ~ @ D\l.." 66:::::":  ! "" # $ % & ' ( @) * "+ , - @. / @0 1 @2 3 @4 5 "6 7 8 @9 : @; ~ @  "~ #? #~ $@ $$ % ~ &@ && ' ~ (@ (( ) +~ , ? ,~ -@ -- . ~ /@ // 0 ~ 1@ 11 2 ~ 3@ 33 4 6~ 7? 7~ 8@ 88 9 ~ :@ :: ; <:":": : : : : :":>@ ()   $%    :;  &' /0 12 34 89 -.  7gg@  0S  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"dXX??cU} } }  } } *} * 0w";#";;;;  ;  ; ;;;;;;;;;;;;  !  ! ((              0; %Z :B; %Z :B; %Z :B; %Z" :"B;* +%Z :B   I !!! J ""       ~ ?;' %Z  : B'~ @;' %Z  : B'   I !!! J ""   #$  K~ ?%@ )Z &; %Z  : B; %Z  : B~ @%@ Z &; %Z ! : !B; %Z ! : !B~ @%@ Z )&; %Z - : -B; %Z - : -B I !!! J ""       6D( l&:&D^]D&:kkD&VD& ;!;";#$;%&;'(;);*;+;,-;./; ~ ?; v H%Z :B; w~ %Z :B; x %Z :B; y %Z :B; x %Z :B !~ !@;!v !%Z :B;!w !%Z :B;!x !%Z :B;!y !%Z :B;!x "%Z :B!"~ "@;"v "%Z :B;"w "%Z :B;"x  "%Z :B;"y %Z :B;"x !%Z :B"#$ $I $!!! $J $"" &&'( ( ( ()~ )?;)z %Z :B )*~ *@;*z "%Z$ :$B *+~ +@;+z  *%Z0 :0B +,- -I -!!! -J -"".  / /! /!!! /! /  $,]]]D&6gggD    )  ! B-         e  ! ! ! ! ! " " " " U""  ) * >@  $$$$////----7gg@  4(/  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"dXX??cU} }  } *4  w  ;7  ;         @  ;                 F p;!!!!!q :     $< ))1?1@1@ )=)rrr )>)rrr )?)rrr  )@ )rrr  )A )rrr  )B )rrr  )C )rrr  +H ,P sn$PH:D %? DBP s( :D %? DBP s, :D %? DB $D 3/.... 1 1 n' 1E 1~ o@ i20 /%L L2Bm45+L LLB~ o@ i28m<~ o@ i2@mD~ o@ i2HmL~ o@ i2PmT~ o@ i2Xm\ .G/?m`)D %BB 4**** 4 --  37.... 1 1 n' 1E 1~ o@ i2d/#%L L2Bmh$5#+L LLB~ o@ i2lmpD+ l,@&>*******& FZZZZZ[F*P ! " # $ % & ' ( ) * + , - . / 0 1 2 3  ~ o@ i 2t  mx!~ !o@ !i!2|!!m "~ "o@ "i"2""m!#~ #o@ #i#2##m"$ $ $.G$/?$m#)D %#BB& &4&**** &4 &-- (38(.... )1 )1 )n' )1E )1~ *o@ *i*2**/*/%L L2B*m0*5*/+L LLB~ +o@ +i+2+*+m*~ ,o@ ,i,2,*,m+*~ -o@ -i-2-*-m,*~ .o@ .i.2.*.m-*~ /o@ /i/2/*/m.* 0.G0/?0m/)D* %*/BB2 242**** 24 2-- ,h|ddddsF FZZZZZ[F.  -** >@22 &&  $$0022   &&    7gg@  ,i,sv  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"dXX??cU} * } }  } }  ,  w " ;# " ; "      " ;#  ; " @     " ;#  ; "      "  ! ! ((          ~ ? ~ @ ~ @  ~ @    0000   1     22 22  ~ @6688 ~  @5577 ~ "@5577 ~ $@3477 0000   1           ~ ? ~ @ ~ @ ~ @D l(><v<<<<F<n<<<<F<z<<<< ;#! " ;# "$ @% & ' ( ) "* ;#+ "  0000   1 "" ""#$ $ $ $2$2 $2$2 $$% %~ %@%6688& &~ & @&5577' '~ '"@'5577( (~ ($@(5577)* **0000 * * 1NF<n<<<<F> @ **((((** &&&&''''$$$$%%%%  7gg@    dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"dXX??U} } w";#"      UUUUUUUUUUV !WWWWWW ! XX  YYYYYYY  ZZZZZZZ  ZZZZZZZ [ ZZZZZZZ ZZZZZZZ 1 \\\\\\\\\  ZZZZZZZ~ 1? ] ]]]]]]]]  ZZZZZZZ~ 1@ ] ]]]]]]]]  ZZZZZZZ~ 1@ ] ]]]]]]]]  ZZZZZZZ~ 1@ ]]]]]]]]]  ZZZZZZZ~ 1@ ] ]]]]]]]]  ZZZZZZZ~ 1@ ]]]]]]]]]  ZZZZZZZ~ 1@ ]]]]]]]]]  ZZZZZZZ~ 1 @ ]]]]]]]]]  ZZZZZZZ~ 1"@]]]]]]]]]  ZZZZZZZ~ 1$@ ]]]]]]]]]  ZZZZZZZ*h,@&&&NNNNBNNNNB>@r             7gg@  "VR  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dll_getDeviceSerialNumberWHPPInstallTimeConfighppdvq01.dll_getDevicePPMDuplexerWResolution600dpiOrientationPORTRAITHPOrientRotate180FalsePaperSizeLETTERMediaTypeAUTOCollateOFFDuplexNONEHPPCompositeUSBFilehppdvq01.dll_getDeviceSerialNumber_getDevicePPMDuplexerHPXMLFileUsedhpc20155.xmlEconomodeFalseHalftoneHPDitherMatrixTextAsBlackFalseTTAsBitmapsSettingTTModeOutlineGraphicsModeRASTERMODERETChoiceTruePrintQualityGroupPQGroup_1AlternateLetterHeadFalseHPDocUISUITrueHPPngPassthroughTrueHPDocPropResourceDatahpzhl4wm.cabHPColorModeMONOCHROME_MODEHPPDLTypePDL_PCL5HPMaxResolutionPDM_600DPIHPPJLEncodingUTF8HPJobAccountingHPJOBACCT_JOBACNTHPBornOnDateHPBODHPJobByJobOverrideJBJOHPSmartDuplexSinglePageJobTrueHPSmartDuplexOddPageJobTrueHPManualDuplexDialogItemsInstructionID_01_FACEDOWN-NOROTATEHPManualFeedOrientationFACEDOWNHPOutputBinOrientationFACEDOWNHPManualDuplexDialogModelModelessHPManualDuplexPageOrderEvenPagesFirstHPMapManualFeedToTray1TrueHPPrintOnBothSidesManuallyFalseHPStraightPaperPathFalseHPNUseDiffFirstPageChoiceTRUEHPPageExceptionsFileHPZPE4wmHPPageExceptionsInterfaceShowPageExceptionsHPPageExceptionsLowEndHPPageExceptionsLowEndVerHPPageExceptionsCoverInsertionHPDuplicateJobNameOverrideSWFWPSAlignmentFileHPZLS4wmPSServicesOptionHLPWithLightsHPSmartHubInet_SID_263_BID_276_HID_265HPSmartHub_SupportandtroubleshootingTRUEHPSmartHub_ProductmanualsTRUEHPSmartHub_CheckfordriverupdatesTRUEHPSmartHub_OrdersuppliesTRUEHPConsumerCustomPaperTrueHPEnableRAWSpoolingTrueHPCustomDuplexableRange3x5_8.5x14HPPaperSizeALMConstraintsEXECUTIVEHPPaperSizeDuplexConstraintsEXECUTIVEHPMediaTypeDuplexConstraintsCARDSTOCK,IUPHdLetter o [none] [none]Arial4Pd?<Automatic>R 44  General Everyday PrintingdMicrosoft Office Excel<vDomain Users;GTP - QC Core;GTP - eGenes Folder;Medfiles Users;GTP - GTP;GTP - Zoltick Folder;GTP - Wilson LabEXCEL.EXEC:\Program Files\Microsoft Office\OFFICE11\EXCEL.EXEIPACDRWDSGATCAPI;F4E8Q;Ki݅&E%9xPPBHAERP"dXX??cU} * 7} U 7} *7} U7} 7} U 7"  w 8 ;9 ; ;9 ;9 ;9 ;9 ; ;  ; ;9;9;9;9;9;8;9;;;9; 99999999 : : (( p    ~ ? ~ @ ~ @  99~ @ 99 99  : :? S)D % BB 99 8   9  000    7777777777777777 p777777777777777     77 99~ ?77~ @77 99~ @  99~ @   ::? )D %BB ;89  000    4$(<80yD&F0",,w 8! ;9(     >@J  7gg@  00DP  dMbP?_*+%&?'?(?)?M HP LaserJet P2015 Series PCL 56C odXXLetterDINU" ,bq<SMTJHP LaserJet P2015 Series PCL 5eInputBinFORMSOURCERESDLLUniresDLLHPPCompositeUSBGetSerialNumberhppdvq01.dl